Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis

Figure 3

EXD treatment promotes self-renewal and osteoblastic differentiation of bMSCs from OVX mice. (a, b) BMSCs were harvested from mice of Sham, OVX and EXD groups and cultured for 4 or 7 days ex vivo. The results demonstrated that OVX reduced the number of spontaneously formed CFU-F but increased that of CFU-Adipo, indicating OVX induced adipogenic differentiation of bMSCs. EXD maintained the number of both CFU-F and CFU-Adipo as the similar level of sham mice (×120). (c, d) At the 7th day of culture, the ALP-positive staining of OVX bMSCs was decreased versus bMSCs from sham group. EXD dramatically increased the level of ALP-positive staining as compared to OVX group. The columns represent the means ± SE from three dishes (six mice) per group. versus sham, ^#, ^## versus OVX.

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