Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis

Figure 4

EXD treatment rescues 389 gene expressions involving in ten pathways both in ex vivo and in vitro. (a) Total 26991 genes were detected by microarray. Among them, 389 genes (a ratio of 1.44%) were fold changed > 1.5. These 389 genes were chosen for the hierarchical clustering and principal component analysis (PCA). The samples fell into three major classes, indicating the reliable qualities of microarray. The results showed EXD treatment in OVX mice shifted the gene expression profile toward the sham mice. (b) The pathways analysis demonstrated ten pathways overlapped between ex vivo and in vitro studies. (c) Quantification of qPCR results from ex vivo experiment. The columns represent the means ± SE () per group. , versus sham, ^#, ^## versus OVX.

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