Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors

Figure 2

In vitro validation of hMultistem transduction. (a) Assessment of firefly luciferase (fLuc) expression via BLI showed a significant difference between transduced (eGFP-fLuc as well as eGFP-fLuc-HSV-tk) and wt hMultistem. (b) GCV killing experiment for validation of HSV-tk expression. Left: BLI signal was significantly decreased in eGFP-fLuc-HSV-tk expressing cells but not in eGFP-fLuc expressing control cells for all GCV concentrations compared to cells without GCV treatment. Right: the BCA protein assay confirmed BLI results and showed a dose-dependent cell killing in eGFP-fLuc-HSV-tk expressing cells but not in eGFP-fLuc expressing cells. Levels of significance are expressed as , , , and .

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