Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors

Figure 4

In vitro and in vivo validation of hU87 transduction with a mCherry-rLuc encoding vector. (a) In vitro analysis of mCherry-rLuc expressing hU87 cells showing sufficient expression of rLuc for in vitro detection by BLI using coelenterazine-h as a substrate. Furthermore, results show no statistically significant signal intensity differences after exposure of the rLuc positive hU87 cells to D-luciferin. (b) MRI-based tumor volume measurements from hU87 tumor bearing animals showed rapid growth with tumors reaching an average tumor size of  mm³ at day 21 after injection. (c) BLI measurements on mCherry-rLuc positive hU87 tumor bearing mice also indicated tumor growth over time. Levels of significance are expressed as , , , and .

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