Differentiation of SMINS-TTF-3 cells in vivo. SMINS-TTF-3 cells infected with lentiviral EGFP vectors were injected into the lateral ventricle of brain in nude pups at the age of 3 days and the brains were collected at 6-week point. (a-b) Some injected SMINS-TTF-3 cells remained as neural stem cells, as indicated by neural stem cell marker Sox2/GFP+. Some cells kept the ability of proliferation, as shown by Ki67/GFP+ staining. (c–f) The injected cells differentiated into astrocytes (GFAP/GFP+), neurons (Map2/GFP+ and NeuN/GFP+), and oligodendrocytes (Olig2/GFP+) in vivo. DAPI was used for nuclei counterstaining (blue). Scale bar: 50 μm. The arrows direct positive cells.