Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules

Figure 4

Differentiation of SMINS-TTF-3 cells in vivo. SMINS-TTF-3 cells infected with lentiviral EGFP vectors were injected into the lateral ventricle of brain in nude pups at the age of 3 days and the brains were collected at 6-week point. (a-b) Some injected SMINS-TTF-3 cells remained as neural stem cells, as indicated by neural stem cell marker Sox2/GFP⁺. Some cells kept the ability of proliferation, as shown by Ki67/GFP⁺ staining. (c–f) The injected cells differentiated into astrocytes (GFAP/GFP⁺), neurons (Map2/GFP⁺ and NeuN/GFP⁺), and oligodendrocytes (Olig2/GFP⁺) in vivo. DAPI was used for nuclei counterstaining (blue). Scale bar: 50 μm. The arrows direct positive cells.

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