Lifespan Extension and Sustained Expression of Stem Cell Phenotype of Human Breast Epithelial Stem Cells in a Medium with Antioxidants

Figure 5

Western blot analysis of Oct-4, CK18, CK19, EMA, maspin, and CK14 expression in HBECs. Lane 1: Type II HBEC30 developed in the MSU-1 medium; Lane 2: Type I HBEC30 developed in the MSU-1 medium; Lane 3: Type I HBEC30 developed in the K-NAC medium (cpdl = 27); Lane 4: Type II HBEC31 developed in the MSU-1 medium; Lane 5: Type I HBEC31 developed in the MSU-1 medium; Lane 6: Type I HBEC31 developed in the K-NAC medium (cpdl = 18); Lane 7: Type I HBEC31 developed in the K-NAC medium (cpdl = 24); Lane 8: Type I HBEC31 developed in the K-NAC medium (cpdl = 29).