Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment
Table 2
Polyamine levels in cells from control, OGD, and OGD + hBM-MSCs groups for 72 h.
Number
Compound
Polyamine levels (ng, mean ± SD) in cells (4 × 105)
Control group ()
OGD group ()
OGD + hBM-MSCs group ()
value
1
N1-Acetylputrescine
ND
ND
ND
2
N1-Acetylcadaverine
ND
ND
ND
3
Putrescine
16.4 ± 1.5
21.8 ± 0.9 (0.002)
28.1 ± 0.9 (0.0003)
0.001
4
Cadaverine
12.5 ± 0.2
12.4 ± 0.4 (0.794)
12.6 ± 0.3 (0.861)
0.501
5
N1-Acetylspermidine
45.6 ± 1.8
66.5 ± 0.8 (0.0003)
54.3 ± 4.7 (0.026)
0.006
6
N8-Acetylspermidine
64.4 ± 0.6
56.7 ± 4.0 (0.047)
50.1 ± 3.3 (0.03)
0.082
7
Spermidine
132.5 ± 3.0
212.1 ± 16.7 (0.0002)
180.4 ± 6.9 (0.004)
0.024
8
N1-Acetylspermine
63.4 ± 6.0
127.3 ± 9.7 (0.0005)
110.6 ± 0.6 (0.0005)
0.050
9
Spermine
64.9 ± 6.2
243.8 ± 15.1 (0.0002)
177.6 ± 9.5 (0.0003)
0.001
One-way ANOVA, value < 0.05 between control and OGD groups. One-way ANOVA, value < 0.05 between control and OGD + hBM-MSCs groups. One-way ANOVA, value < 0.05 between OGD and OGD + hBM-MSCs groups. Not determined.
