3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis

<div>Morphology and paracrine secretions of human adipose-derived MSCs. (a) Morphology of 2D cultured MSCs and 3D spheroids of MSCs (bar = 100 <i>μ</i>m). (b) Viability of 3D spheroid-derived MSCs. 3D spheroid-derived MSCs were stained with PI and analyzed by flow cytometry, indicating that the cell viability is over 90%. (c) Paracrine secretions of MSCs. The mRNA levels of IGF-1, HGF, and IL-6 in 3D spheroids of MSCs of different days and 2D cultured MSCs were determined by RT-PCR. GAPDH was the internal control. (d) The protein levels of IGF-1, HGF, and IL-6 in the conditioned medium of 2D cultured MSCs and 3D spheroids of MSCs were determined by ELISA.</div>

Stem Cells International

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Figure 1

Figure 1: 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis 

Figure 1 | 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis 