Mesenchymal Stem Cells Derived from Dental Pulp: A Review

Table 2

Transplantation assays using MSCs obtained from human tooth sources (DPSCs & SHED cells).

Source/scaffold	Host	Cells	Weeks	Results	Reference
DPSC/HA-TCP	I.Mice	5 × 10^6	6	Formation of pulp, dentine, and odontoblast-like tissue	Gronthos et al. [53]
DPSC/HA/TCP	I.Mice & swine	4 × 10^6	12	Formation of pulp tissue, dentine, and cementum	Sonoyama et al. [20]
DPSC/NF-PLA	I.Mice	1 × 10^6	8	Generation of odontoblast-like cells and a collagen-like matrix	Wang et al. [56]
DPSC/fibrin	Mice	1 × 10^6	4	Formation of dentine-like tissue	Chun et al. [57]
DPSC/NF	I.Mice	1 × 10^5	4	Mineralized tissue formation	Chen et al. [2]
DPSC/chitosan/collagen	I.Mice	1 × 10^6	4	Mineralized tissue formation	Yang et al. [33]
DPSC/HA/TCP	I.Mice	5 × 10^7	12	Formation of dentine-like matrix	Sun et al. [58]
DPSC-3M/HA/TCP	I.Mice	4 × 10^6	2, 4, 8, and 16	Differentiation into osteoblasts & odontoblast-like cells on the surface of HA/TCP scaffold	Batouli et al. [59]
DPSC-3M/ TCP	SCID mice	1 × 10^6	8–15	Generation of dentine pulp-like structure	Takeda et al. [60]
DPSC-3M/PLGA	I.Rats	1 × 10^6	4–8	Bone nodule formation	Graziano et al. [61]
DPSC-3M	Nude rats	1 × 10^6	2–4	Improvement in cardiac function, angiogenesis, and reduced myocardial infarct size	Gandia et al. [62]
DPSC-3M/HA	I.Mice	1 × 10^6	8	Mineralized tissue formation	Ikeda et al. [63]
DPSC-3M/PLGA	SCID mice	1 × 10^7	12–16	Formation of pulp-like tissue	Huang et al. [52]
DPSC-3M/dentine	I.Mice	1 × 10^6	4	Mineralized tissue formation	Demarco et al. [64]
DPSC-3M/Ceramic/bone	I.Mice	5 × 10^6	6	Formation of dentine, pulp-like tissue	Wang et al. [56]
DPSC-3M/HFIP/silk	I.Mice	4.5 × 10^5	6, 18, and 25	Formation of soft pulp-like tissue	Yang et al. [33]
DPSC-3M/HA/TCP	I.Mice	1 × 10^7	6 & 12	Formation of pulp-like structure lined with odontoblast-like cells	Lee et al. [65]
DPSC-3M/fibroin	Sprague-Dawley rats	1500/mm^3	4	Bone formation	Riccio et al. [66]
DPSC-3M	I.Mice	—	8	Generation of pulp-like & periodontal ligament type tissue	Lei et al. [67]
DPSC-3M/dentine	I.Mice	1 × 10^4	4, 6, and 8	Mineralized tissue and dentine formation	Tran and Doan [68]
DPSC-3M & SHED	Avian embryos	5 × 10^3	24, 48, and 72 h	Neuronal marker expression and neuronal morphogenesis	Arthur et al. [69]
DPSC-3M & SHED	Sprague-Dawley rats	1 × 10^6	8	Axonal regeneration and inhibition of apoptosis in neurons, astrocytes, and oligodendrocytes	Sakai et al. [46]
SHED/collagen	Wistar rats	1 × 10^6	1, 3, 4, and 8	Dense and mature bone formation	de Mendonça Costa et al. [70]
SHED/PLA	SCID mice	5 × 10^6	3	Solid training in dental tissue samples	Sakai et al. [46]
SHED/FPOL	I.Mice	5 × 10^5	5	Formation of vascularized connective tissue	Galler et al. [71]
DPSC-3M/collagen	Human	—	52	Formation of bone in the extracted socket and repair of periodontal tissue	D’Aquino et al. [51]
DPSC-3M/collagen	Human	—	186	Repair of human mandible bone defects with compact and uniformly vascularized bone	Giuliani et al. [72]

DPSCs: derived from permanent teeth; 3M: third molar; SHED: derived from human exfoliated deciduous teeth; I.Mice: immunodeficient mice; SCID: severe combined immunodeficiency; FPOL: PEGylated fibrin; NF: nanofibrous: PLA: poly(L-lactic-acid); HFIP: hexafluoro-2-propanol; PLG: poly-D,L-lactide and glycolide; HA/TCP: hydroxyapatite/tricalcium phosphate; AT: autologous transplant.