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Stem Cells International
Volume 2016 (2016), Article ID 4729535, 11 pages
http://dx.doi.org/10.1155/2016/4729535
Research Article

Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4

1Department of Cell Biology and Stem Cell Research Center, School of Basic Medicine, Peking University, Beijing, China
2Baotou Medical College, Baotou, Inner Mongolia, China
3Beijing Cellapy Biotechnology Co., LTD, Beijing, China
4Department of Pharmaceutics, University of Florida, 6550 Sanger Road, Orlando, FL 32827, USA
5Beijing Anzhen Hospital, Beijing, China

Received 5 August 2016; Accepted 1 September 2016

Academic Editor: Gary E. Lyons

Copyright © 2016 Shixin Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Induced pluripotent stem (iPS) cells have been generated from human somatic cells by ectopic expression of four Yamanaka factors. Here, we report that Survivin, an apoptosis inhibitor, can enhance iPS cells generation from human neural progenitor cells (NPCs) together with one factor OCT4 (1F-OCT4-Survivin). Compared with 1F-OCT4, Survivin accelerates the process of reprogramming from human NPCs. The neurocyte-originated induced pluripotent stem (NiPS) cells generated from 1F-OCT4-Survivin resemble human embryonic stem (hES) cells in morphology, surface markers, global gene expression profiling, and epigenetic status. Survivin keeps high expression in both iPS and ES cells. During the process of NiPS cell to neural cell differentiation, the expression of Survivin is rapidly decreased in protein level. The mechanism of Survivin promotion of reprogramming efficiency from NPCs may be associated with stabilization of β-catenin in WNT signaling pathway. This hypothesis is supported by experiments of RT-PCR, chromatin immune-precipitation, and Western blot in human ES cells. Our results showed overexpression of Survivin could improve the efficiency of reprogramming from NPCs to iPS cells by one factor OCT4 through stabilization of the key molecule, β-catenin.