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Stem Cells International
Volume 2016, Article ID 4834616, 12 pages
http://dx.doi.org/10.1155/2016/4834616
Research Article

Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

1Department of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USA
2Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, Germany
3Department of Chemical Engineering, Kansas State University, 1005 Durland Hall, Manhattan, KS 66506, USA
4Faculty of Biology and Chemistry, Justus Liebig University, Ludwigstraße 23, 35390 Gießen, Germany
5Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Project Group Bioresources, Winchester Straße 2, 35392 Gießen, Germany

Received 7 October 2015; Revised 28 December 2015; Accepted 29 December 2015

Academic Editor: Renke Li

Copyright © 2016 Florian Petry et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.