Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

Table 2

Maximal growth rate, minimum doubling time, and fold expansion of hUC MSCs from five donors, after 7 days in culture in 1 mL DMEM LG medium in a 12-well plate at a seeding density of 8000 cells⋅cm⁻² ().

HUC #	Gender	Max. growth rate (h^−1)	Min. doubling time (h)	Fold expansion
255	Female	0.045 ± 0.002	15.4 ± 0.6	12.7
260	Female	0.049 ± 0.002	18.1 ± 1.3	11.0
262	Female	0.037 ± 0.004	17.3 ± 1.7	10.9
256	Male	0.038 ± 0.003	14.2 ± 0.5	11.1
257	Male	0.040 ± 0.004	18.8 ± 2.1	11.2
Average		0.042	16.8	11.4
Standard deviation		0.005	1.9	0.7