Research Article

Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

Table 3

Growth characteristics for hUC MSCs on Plastic Plus microcarriers in spinner flasks with 100 mL DMEM LG culture medium at a seeding density of 8000 cells⋅cm−2.

GenderCulture time (d)Max. growth rate   (h−1)Min. doubling time    (h)Fold expansionHighest occupancy (%)Harvested cell yield

Male70.046 ± 0.00614.9 ± 2.216.4812.6 107
Female60.032 ± 0.00321.6 ± 2.513.8935.3 107