Human Amnion-Derived Mesenchymal Stem Cells Promote Osteogenic Differentiation in Human Bone Marrow Mesenchymal Stem Cells by Influencing the ERK1/2 Signaling Pathway

Figure 2

The effect of HAMSCs on HBMSC proliferation was measured by flow cytometry and immunofluorescence staining of 5-ethynyl-2′-deoxyuridine (EdU). (a) Cell cycle fractions (G0, G1, S, and G2 M phases) at 3, 6, and 12 d. (b) Immunofluorescence staining of EdU at 12 d. (c) Area of stained EdU-positive nodules relative to the total culture surface at 12 d was measured by Image-Pro Plus (IPP) analysis. Scale bar: 100 μm, , and . Control: HBMSCs cultured without HAMSCs; HBMSCs cocultured with HAMSCs; H1: HBMSC : HAMSC = 1 : 1; H2: HBMSC : HAMSC = 1 : 2; H3: HBMSC : HAMSC = 1 : 3.

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