Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation

Figure 2

3D cultured NPCs form proliferating neurospheres positive for PAX6 and NESTIN. (a) Phase contrast images of neurospheres from all 3 lines (N S5, N S8, and RT S11) on day 4 and day 7. Scale bar = 100 μm. (b) Analyses of neurosphere diameter on day 4 and day 7 of 3D suspension culture. Significant difference was tested via one-way ANOVA analysis with a Tukey post hoc multiple comparison test. Significant differences were indicated as follows: and . Results were reported as Mean ± SEM of 9 fields from 3 independent cultures. (c) ICC staining of neurospheres (NESTIN, red, and PAX6, green) on day 7 from all 3 lines. Scale bar = 100 μm. (d) Representative ICC of 2D NPCs (KI67). Scale bar = 100 μm. (e) Quantification of KI67⁺ NPCs for 2D culturing systems. Results were reported as Mean ± SEM of 15 fields from 3 independent cultures, tested for significant differences () via one-way ANOVA analysis with a Tukey post hoc multiple comparison test.

(a)

(b)

(c)

(d)

(e)