Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation

<div>Comparison of NPC markers expression in 2D and 3D cultures. Both 2D and 3D NPCs were dissociated into single cells and plated onto POL/L coated coverslips. (a) Representative ICC of 2D NPCs and 3D NPCs (PAX6 and NESTIN). Scale bar = 100 <i>μ</i>m. (b) ICC of 2D NPCs and 3D NPCs (Musashi-1 and SOX2). Scale bar = 100 <i>μ</i>m.</div>

Stem Cells International

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Figure 3

Figure 3: Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation 