Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation

Table 1

Human specific primers used for qPCR analysis.

Symbol	Sequence 5′-3′	Product size (BP)	Gene name	Reference number (NCBI)	Reference
GAPDH	GGTCACCAGGGCTGCTTTTA; GGATCTCGCTCCTGGAAGATG	195	Glyceraldehyde-3-phosphate dehydrogenase	NM_001289746.1	[40]
NESTIN	CTCCAGAAACTCAAGCACC; TCCTGATTCTCCTCTTCCA	145	Nestin	NM_006617.1	[41]
SOX2	TTCACATGTCCCAGCACTACCAGA; TCACATGTGTGAGAGGGGCAGTGTGC	80	SRY- (sex determining region Y-) box 2	NM_003106.3	[42]
PAX6	TGGTATTCTCTCCCCCTCCT; TAAGGATGTTGAACGGGCAG	126	Paired box 6	XM_005252958.3	[43]
BLBP	GGCTTTGCCACTAGGCAGG; TGACCACTTTGTCTCCTTCTTGA	76	Brain lipid-binding protein	XM_005266858.2	[44]
TUBB3	TCCGCTCAGGGGCCTTTGGAC; GCTCCGCCCCCTCCGTGTAG	108	Tubulin, beta 3 class III	NM_001197181.1
TBR2	CACCGCCACCAAACTGAGAT; CGAACACATTGTAGTGGGCAG	109	Eomesodermin	NM_001278182.1	[45]
AQP4	AGCAGTCACAGCGGAATTTCT; TCTGTTCCACCCCAGTTGATG	81	Aquaporin 4	XM_011525942.1	[44]
MAP2	AGTTCAGGCCCACTCTCCCTCC; GGGAGCCAGAGCTGATTCCCCA	127	Microtubule-associated protein 2	XM_011511198.1
GFAP	AGGTCCATGTGGAGCTTGAC; GCCATTGCCTCATACTGCGT	82	Glial fibrillary acidic protein	NM_001242376.1	[44]