Adipose Derived-Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

Figure 7

ATD-MSCs differentiating abilities in vitro were preserved at both temperatures of cryopreservation. After cell culture, ATD-MSCs assumed the typical fibroblast-like morphology (a) and expressed CD105, CD44, CD73, and CD271 (b–e). The immunophenotype of MSCs expressing CD105/CD44/CD73 and of the subpopulation CD105/CD44/CD73/CD271+ cells was comparable with both the temperatures of cryopreservation (f, g). Chondrocytes (h, i) and osteoblasts (j, k) formed from both adipose tissues cryopreserved at −80°C and –196°C. von Kossa staining showed that osteoblasts had comparable ability to mineralize at both temperatures (l, m).

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