The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease

Figure 1

The expression of FGF-20 in transduced hUC-MSCs in vitro. (a) Expression of GFP in hUC-MSCs transduced by Ad-GFP as determined by fluorescence microscopy. (b) Flow cytometric analysis of GFP expression in UCMSC transduced by Ad-GFP. (c) Production of bioactive FGF-20 in the supernatant of hUC-MSCs. Supernatant from cultured cells was retrieved on the indicated time points, processed, and analyzed using a mouse specific FGF-20 ELISA assay. (d) Typical bands of FGF-20 and GAPDH detected by western blotting. (e) Gray intensity analysis of FGF-20. All data are presented as mean ± standard error of mean (, hUC-MSCs versus hUC-MSC-FGF-20 and UCMSC-GFP; , hUC-MSCs versus hUC-MSC-FGF-20).

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