Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms

Figure 3

Expression of Oct-4A and Nanog in selected BM populations on protein level. Representative images of Oct-4A- and Nanog-expressing CD45⁻/Lin⁻/CD106⁺ cells by ImageStream X system. TNCs were stained for (1) Oct-4A (Alexa Fluor 488, green), CD45 (PE-Cy7, magenta), and CD106 (PE, orange) (a) and (2) Nanog (Alexa Fluor 647, blue), Lin markers (FITC, green), CD45 (PE-Cy7, magenta), and CD106 (PE, orange) (b). Cell were stained with Hoechst 33342 to visualize nuclei and analyzed by ISS to detect intranuclear expression of Oct-4A (a) and Nanog (b) as shown in magnified, combined images. The scale bars indicate 10 μm.

(a)

(b)