Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms

<div>Expression of Oct-4A and Nanog in sorted fractions of rVSELs (CD45<sup>−</sup>/Lin<sup>−</sup>/CD106<sup>+</sup>) and rHSCs (CD45<sup>+</sup>/Lin<sup>−</sup>/CD106<sup>+</sup>) by immunocytochemistry. Both fractions were sorted with MoFlo XDP cell sorter (Beckman Coulter) and further stained for Oct-4A (Alexa Fluor 488, green) (a) or Nanog (Alexa Fluor 546, red) (b) and analyzed with Leica DMI6000B (ver. AF7000) fluorescent microscope. Nuclei are stained with DAPI. Intranuclear staining for both transcription factors is visualized on combo images. The scale bars indicate 10 <i>μ</i>m.</div>

Stem Cells International

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Figure 5: Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms 

Figure 5 | Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms 