Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Figure 3

Osteogenic differentiation potential of third-passage MSCs cultures derived from meniscal debris. After 14-day induction in osteogenic medium, osteogenesis was determined based on deposition of matrix calcification detected by Alizarin Red (a) and based on ALP-specific staining (b). Scale bar 100 μm. Expression of osteogenic genes encoding Runx2 (c), ALP (d), OCN, (e) and type I collagen (f) was assessed using real-time PCR. Data are presented as mean ± SD. .

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