Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Figure 4

Adipogenic differentiation potential of third-passage MSCs cultures derived from meniscal debris. After 14-day induction in adipogenic medium, adipogenesis was detected as the formation of neutral lipid vacuoles (a) stainable with Oil Red O (b). Scale bar = 200 μm. Expression of adipogenic genes PPARγ (c), adiponectin (d), LPL (e), PAS (f), and aP2 (g) was assessed by real-time PCR. Data are presented as mean ± SD. ; .

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