Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Figure 5

Chondrogenic differentiation potential of third-passage MSCs cultures derived from meniscal debris. At 21 day after chondrogenic induction, chondrogenesis was induced under serum-free micromass pellet culture conditions (a) and analyzed using hematoxylin and eosin staining (b) for general histology, Toluidine Blue (c) and Alcian Blue staining (d) for GAG, and immunohistochemical staining for type II collagen in chondrogenic matrix ((e): low-magnification; (f): high-magnification). Scale bar = 100 μm. Expression of chondrogenic genes SOX9 (g), type II collagen (h), and GAG (i) was assessed by real-time PCR. Data are presented as mean ± SD. ; .

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