Mesenchymal Stem/Stromal Cells from Discarded Neonatal Sternal Tissue: In Vitro Characterization and Angiogenic Properties

Figure 7

Conditioned media from hypoxic neonatal sbMSCs promote HUVEC migration and proliferation. (a) HUVEC migration was assessed by a scratch assay. Conditioned media from all hypoxic sbMSCs lines enhanced and accelerated scratch healing as assessed by average (error bars = standard deviation) wound width, density, and confluence as compared to basal media, EGM-2. Growth factor supplemented EGM-2(+) was used as a positive control. Data were analyzed by one-way ANOVA with post hoc Tukey test and are representative of two independent experiments. (b) HUVEC proliferation was assessed by the MTT assay. Conditioned media from all hypoxic sbMSCs lines enhanced HUVECs proliferation as compared to basal EGM-2 (, ). Data were analyzed by one-way ANOVA with post hoc Tukey test and are representative of two independent experiments.

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