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Stem Cells International
Volume 2016, Article ID 5127984, 11 pages
Research Article

Inhibition of JAK-STAT ERK/MAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem Cells

1CSIRO Health & Biosecurity, East Geelong, VIC 3219, Australia
2Monash Institute for Medical Research, Monash University, Clayton, VIC 3168, Australia
3Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
4Department of Materials Engineering, Monash Institute of Medical Engineering, Faculty of Engineering, Monash University, Clayton, VIC 3168, Australia
5South Australian Research & Development Institute (SARDI), Turretfield Research Centre, Rosedale, SA 5350, Australia

Received 13 July 2015; Revised 25 October 2015; Accepted 29 October 2015

Academic Editor: Tao-Sheng Li

Copyright © 2016 Luis F. Malaver-Ortega et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Alkaline phosphate activity in the biPSC colonies produced using alkaline phosphatase activity was determined using the Alkaline Phosphatase Detection Kit (Millipore, SCR004) following manufactures’ instructions. The colonies were weakly positive. All the cell lines displayed normal karyotypes corresponding to male individuals.

Table S1: The list of primers used during the study is presented. The primers were selected to distinguish between endogenous and ectopic gene expression of the genes evaluated.

  1. Supplementary Material