Figure 2: PEDF in different passages of rat ADSC-conditioned medium. Five samples per passage were analyzed using a rat PEDF ELISA kit. The different passages of the rat ADSCs secreted a detectable level of PEDF in cultured serum-free DMEM/F12 medium. compared with the DMEM/F12 medium group only. PEDF = pigment epithelium-derived factor; ADSCs = adipose tissue-derived stem cells; DMEM/F12 = Dulbecco’s Modified Eagle’s Medium/F12.