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Figure 4: Western blotting analyses of PEDF, p-Akt, and p-eNOS protein expression in the rat penile tissues of all groups. (a) Representative Western blot and (b) quantitative analysis identified increased PEDF expression in the bilateral cavernous nerve injury (BCNI) group compared with the sham group at day 14, which recovered to the background level at day 28. . (c)-(d) Increased PEDF expression in the ADSCs treatment group compared with the other groups at day 28. . (e)-(f) Increased p-Akt expression in the ADSCs group compared with the other groups at day 28 and increased p-Akt expression in the sham group compared with the bilateral cavernous nerve injury and PBS groups. and . (g-h) Increased p-eNOS expression in the ADSCs treatment group compared with the other groups at day 28. . The data were normalized to GAPDH protein expression. per group. p-Akt = phosphorylated Akt; p-eNOS = phosphorylated endothelial nitric oxide synthase; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.