Research Article
Phenotypic and Functional Characterization of Mesenchymal Stem/Multipotent Stromal Cells from Decidua Basalis of Human Term Placenta
Table 7
The mean expression of the percentage of immune proteins in DBMSCs as determined by flow cytometry.
| | Markers | Passage 3 | Passage 4 | Passage 5 | Markers | Passage 3 | Passage 4 | Passage 5 |
| | IL-1 | — | — | — | D6 | 10.13 ± 2.52 | 13.03 ± 6.28 | 12.30 ± 4.02 | | IL-6 | 59.70 ± 1.03 | 63.00 ± 0.77 | 64.60 ± 5.40 | CCR1 | — | — | — | | IL-8 (CXCL8) | 14.4 ± 2.51 | 10.4 ± 0.51 | 17.4 ± 1.51 | CCR2 | — | — | — | | IL-10 | 50.77 ± 4.08 | 43.43 ± 8.15 | 45.73 ± 2.46 | CCR3 | — | — | — | | IL-12 | 90.00 ± 6.90 | 87.95 ± 7.05 | 71.35 ± 9.25 | CCR4 | — | — | — | | TNF-α | 53.37 ± 2.7 | 53.97 ± 10.95 | 54.00 ± 4.23 | CCR5 | — | — | — | | MCP-1 | 64.47 ± 5.98 | 77.40 ± 7.61 | 81.30 ± 8.20 | CCR6 | — | — | — | | HMGB-1 | 71.33 ± 3.69 | 72.63 ± 12.39 | 65.5 ± 8.72 | CCR7 | — | — | — | | IFN-γ | 53.60 ± 3.24 | 35.10 ± 1.170 | 24.4 ± 2.884 | CCR8 | — | — | — | | IFNAR1 | 48.87 ± 2.63 | 51.50 ± 3.72 | 41.47 ± 4.26 | CCR9 | — | — | — | | IFNAR2 | 73.43 ± 18.09 | 94.10 ± 1.89 | 93.13 ± 2.72 | CCR10 | — | — | — | | TLR3 | 13.13 ± 5.79 | 13.47 ± 3.24 | 17.27 ± 2.83 | CXCR1 | — | — | — | | TLR7 | 8.76 ± 3.06 | 10.43 ± 6.22 | 8.73 ± 2.92 | CXCR2 | — | — | — | | TLR9 | 19.80 ± 3.18 | 16.37 ± 5.03 | 23.13 ± 3.05 | CXCR3 | — | | | | IL5-R | 16.50 ± 1.041 | 9.86 ± 5.13 | 10.43 ± 3.53 | CXCR4 | — | — | — | | IL-7R (CD127) | — | — | — | CXCR7 | — | — | — | | TGF B1 | 74.40 ± 6.01 | 35.60 ± 5.50 | 23.30 ± 2.81 | XCR1 | — | — | — | | TGFB1,2,3 | 37.00 ± 1.07 | 30.03 ± 5.76 | 37.40 ± 5.09 | CX3CR1 | — | — | — | | MMP7 | 51.70 ± 3.85 | 63.73 ± 13.45 | 57.10 ± 2.81 | CXCL1 | 20.07 ± 2.73 | 19.07 ± 5.05 | 25.53 ± 6.98 | | ICAM-1 (CD54) | 62.4 ± 5.234 | 76.6 ± 4.022 | 48 ± 2.609 | CXCL4 | 95.50 ± 1.73 | 97.07 ± 0.94 | 95.67 ± 3.08 | | CD116 | — | — | — | CXCL5 | 7.86 ± 3.32 | 5.63 ± 1.75 | 5.00 ± 1.12 | | CD118 | 14.83 ± 4.95 | 17.03 ± 9.81 | 12.87 ± 4.36 | CXCL9 | 87.67 ± 1.13 | 87.70 ± 8.32 | 92.67 ± 5.55 | | CD120a | 11.27 ± 3.78 | 12.97 ± 8.24 | 4.60 ± 2.01 | IP10 (CXCL10) | 9.40 ± 2.45 | 4.03 ± 1.36 | 3.96 ± 1.15 | | CD120b | 10.57 ± 4.23 | 27.53 ± 20.49 | 5.36 ± 0.39 | CXCL12 | 15.83 ± 2.00 | 13.40 ± 3.80 | 21.33 ± 7.45 | | CD130 | 28.30 ± 4.56 | 50.63 ± 22.36 | 38.33 ± 12.29 | CX3CL1 | 10.30 ± 4.18 | 8.13 ± 2.31 | 6.23 ± 0.44 | | CD134 | 18.43 ± 5.49 | 15.33 ± 6.25 | 11.20 ± 1.05 | CCL5 (RANTES) | 13.93 ± 5.38 | 5.93 ± 2.22 | 6.13 ± 4.04 | | CD234 | 9.96 ± 0.77 | 8.93 ± 4.65 | 7.33 ± 2.31 | CCL11 | 9.90 ± 4.25 | 8.23 ± 4.48 | 6.60 ± 1.55 | | CD273 | 96.83 ± 0.66 | 90.73 ± 6.39 | 86.50 ± 9.95 | | | | | | B7H1 (CD274) | 98.00 ± 0.21 | 91.80 ± 3.63 | 85.60 ± 2.01 | | | | | | CD276 (B7H3) | 99.97 ± 0.03 | 99.93 ± 0.06 | 99.87 ± 0.088 | | | | | | B7H4 | 86.43 ± 1.18 | 72.30 ± 4.29 | 82.10 ± 6.97 | | | | | | IDO | 38.37 ± 3.73 | 31.50 ± 5.90 | 36.97 ± 1.37 | | | | |
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Note. CD130: IL-6 Receptor Associated Transducer; CD116: GMCSF receptor; CD118: Leukemia Inhibitory Factor Receptor; MMP7: Matrix Metalloproteinase 7; D6: Chemokine Decoy Receptor.
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