Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium

Figure 6

Differentiation capacities of hMSC-TERT cells and primary adipose-derived hMSCs (ad-hMSCs). The cells were induced to undergo adipogenic, chondrogenic, or osteogenic differentiation in commercial media after expansion in CDM. Differentiation was confirmed by nil red staining (red, adipogenic), collagen type II immunostaining (green, chondrogenic), or hydroxyapatite staining (green, osteogenic). The primary bone marrow-derived hMSCs (bm-hMSCs) behaved in a similar manner (data not shown).