Table of Contents Author Guidelines Submit a Manuscript
Table of Contents Alerts

To receive news and publication updates for Stem Cells International, enter your email address in the box below.

Stem Cells International
Volume 2016 (2016), Article ID 5246584, 10 pages
http://dx.doi.org/10.1155/2016/5246584
Research Article

Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium

Denise Salzig,1 Jasmin Leber,1 Katharina Merkewitz,1 Michaela C. Lange,1 Natascha Köster,1 and Peter Czermak1,2,3,4

1Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, 35390 Giessen, Germany
2Faculty of Biology and Chemistry, Justus Liebig University, 35390 Giessen, Germany
3Project Group Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35394 Giessen, Germany
4Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA

Received 8 October 2015; Revised 18 January 2016; Accepted 26 January 2016

Academic Editor: Tao-Sheng Li

Copyright © 2016 Denise Salzig et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. T. Opperman, J. Leber, C. Elseberg, D. Salzig, and P. Czermak, “hMSC production in disposable bioreactors in compliance with cGMP guidelines and PAT,” American Pharmaceutical Review, vol. 17, no. 3, pp. 42–47, 2014. View at Google Scholar
  2. K. Cierpka, C. L. Elseberg, K. Niss, M. Kassem, D. Salzig, and P. Czermak, “HMSC production in disposable bioreactors with regards to GMP and PAT,” Chemie-Ingenieur-Technik, vol. 85, no. 1-2, pp. 67–75, 2013. View at Publisher · View at Google Scholar · View at Scopus
  3. A. L. Berrier and K. M. Yamada, “Cell-matrix adhesion,” Journal of Cellular Physiology, vol. 213, no. 3, pp. 565–573, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. M.-H. Disatnik, S. C. Boutet, W. Pacio et al., “The bi-directional translocation of MARCKS between membrane and cytosol regulates integrin-mediated muscle cell spreading,” Journal of Cell Science, vol. 117, no. 19, pp. 4469–4479, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. J. E. Murphy-Ullrich, “The de-adhesive activity of matricellular proteins: is intermediate cell adhesion an adaptive state?” Journal of Clinical Investigation, vol. 107, no. 7, pp. 785–790, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. O.-W. Merten and M. C. Flickinger, “Cell detachment,” in Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, pp. 1–22, John Wiley & Sons, New York, NY, USA, 2009. View at Google Scholar
  7. FDA, FDA Proposes Barring Ceratin Cattle Material from Medical Products As BSE Safeguard, FDA, Silver Spring, Md, USA, 2007.
  8. D. W. Jayme and S. R. Smith, “Media formulation options and manufacturing process controls to safeguard against introduction of animal origin contaminants in animal cell culture,” Cytotechnology, vol. 33, no. 1, pp. 27–36, 2000. View at Publisher · View at Google Scholar · View at Scopus
  9. S. Jung, K. M. Panchalingam, L. Rosenberg, and L. A. Behie, “Ex vivo expansion of human mesenchymal stem cells in defined serum-free media,” Stem Cells International, vol. 2012, Article ID 123030, 21 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  10. S. A. Tarle, S. Shi, and D. Kaigler, “Development of a serum-free system to expand dental-derived stem cells: PDLSCs and SHEDs,” Journal of Cellular Physiology, vol. 226, no. 1, pp. 66–73, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. D. R. Marshak and J. J. Holecek, “Chemically defined medium for human mesenchymal stem cells,” United States Patent 5,908,782, 1999.
  12. C.-H. Liu, M.-L. Wu, and S.-M. Hwang, “Optimization of serum free medium for cord blood mesenchymal stem cells,” Biochemical Engineering Journal, vol. 33, no. 1, pp. 1–9, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. G. Rajaraman, J. White, K. S. Tan et al., “Optimization and scale-up culture of human endometrial multipotent mesenchymal stromal cells: potential for clinical application,” Tissue Engineering Part C: Methods, vol. 19, no. 1, pp. 80–92, 2013. View at Publisher · View at Google Scholar · View at Scopus
  14. D. P. Lennon, S. E. Haynesworth, R. G. Young, J. E. Dennis, and A. I. Caplan, “A chemically defined medium supports in vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stem cells,” Experimental Cell Research, vol. 219, no. 1, pp. 211–222, 1995. View at Publisher · View at Google Scholar · View at Scopus
  15. A. M. Parker, H. Shang, M. Khurgel, and A. J. Katz, “Low serum and serum-free culture of multipotential human adipose stem cells,” Cytotherapy, vol. 9, no. 7, pp. 637–646, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. T. E. Ludwig, V. Bergendahl, M. E. Levenstein, J. Yu, M. D. Probasco, and J. A. Thomson, “Feeder-independent culture of human embryonic stem cells,” Nature Methods, vol. 3, no. 8, pp. 637–646, 2006. View at Publisher · View at Google Scholar
  17. J. E. Hudson, R. J. Mills, J. E. Frith et al., “A defined medium and substrate for expansion of human mesenchymal stromal cell progenitors that enriches for osteo- and chondrogenic precursors,” Stem Cells and Development, vol. 20, no. 1, pp. 77–87, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Mimura, N. Kimura, M. Hirata et al., “Growth factor-defined culture medium for human mesenchymal stem cells,” International Journal of Developmental Biology, vol. 55, no. 2, pp. 181–187, 2011. View at Publisher · View at Google Scholar · View at Scopus
  19. E. F. Plow, T. A. Haas, L. Zhang, J. Loftus, and J. W. Smith, “Ligand binding to integrins,” The Journal of Biological Chemistry, vol. 275, no. 29, pp. 21785–21788, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. C. Niehage, C. Steenblock, T. Pursche, M. Bornhäuser, D. Corbeil, and B. Hoflack, “The cell surface proteome of human mesenchymal stromal cells,” PLoS ONE, vol. 6, no. 5, Article ID e20399, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Docheva, C. Popov, W. Mutschler, and M. Schieker, “Human mesenchymal stem cells in contact with their environment: surface characteristics and the integrin system,” Journal of Cellular and Molecular Medicine, vol. 11, no. 1, pp. 21–38, 2007. View at Publisher · View at Google Scholar · View at Scopus
  22. J. L. Simonsen, C. Rosada, N. Serakinci et al., “Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells,” Nature Biotechnology, vol. 20, no. 6, pp. 592–596, 2002. View at Publisher · View at Google Scholar · View at Scopus
  23. K. Cierpka, N. Mika, M. C. Lange, H. Zorn, P. Czermak, and D. Salzig, “Cell detachment by prolyl-specific endopeptidase from Wolfiporia cocos,” American Journal of Biochemistry and Biotechnology, vol. 10, no. 1, pp. 14–21, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. F. Xu, S. Ito, M. Hamaguchi, and T. Senga, “Disruption of cell spreading by the activation of MEK/ERK pathway is dependent on AP-1 activity,” Nagoya Journal of Medical Science, vol. 72, no. 3-4, pp. 139–144, 2010. View at Google Scholar · View at Scopus
  25. C. Elseberg, D. Salzig, and P. Czermak, “Bioreactor expansion of human mesenchymal stem cells according to GMP requirements,” in Stem Cells and Good Manufacturing Practices, K. Turksen, Ed., vol. 1283, pp. 199–218, Springer, New York, NY, USA, 2015. View at Google Scholar
  26. F. Ehlicke, D. Freimark, B. Heil, A. Dorresteijn, and P. Czermak, “Intervertebral disc regeneration: influence of growth factors on differentiation of human mesenchymal stem cells (hMSC),” International Journal of Artificial Organs, vol. 33, no. 4, pp. 244–252, 2010. View at Google Scholar · View at Scopus
  27. A. M. P. Pardo, M. Bryhan, H. Krasnow et al., “Corning® CellBIND® surface: an improved surface for enhanced cell attachment,” Tech. Rep., 2005. View at Google Scholar
  28. L. J. Foster, P. A. Zeemann, C. Li, M. Mann, O. N. Jensen, and M. Kassem, “Differential expression profiling of membrane proteins by quantitative proteomics in a human mesenchymal stem cell line undergoing osteoblast differentiation,” Stem Cells, vol. 23, no. 9, pp. 1367–1377, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. K. M. Mak, P. Sehgal, and C. K. Harris, “Type VI collagen: its biology and value as a biomarker of hepatic fibrosis,” Austin Biomarkers & Diagnosis, vol. 1, pp. 1–9, 2014. View at Google Scholar
  30. P. Chen, M. Cescon, and P. Bonaldo, “Collagen VI in cancer and its biological mechanisms,” Trends in Molecular Medicine, vol. 19, no. 7, pp. 410–417, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. L. A. Solchaga, K. Penick, J. D. Porter, V. M. Goldberg, A. I. Caplan, and J. F. Welter, “FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells,” Journal of Cellular Physiology, vol. 203, no. 2, pp. 398–409, 2005. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Tsutsumi, A. Shimazu, K. Miyazaki et al., “Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF,” Biochemical and Biophysical Research Communications, vol. 288, no. 2, pp. 413–419, 2001. View at Publisher · View at Google Scholar · View at Scopus
  33. L. P. Roncoroni, J. K. Maerz, B. Angres et al., “Adhesion to extracellular matrix proteins can differentiate between human bone marrow derived mesenchymal stem cells and fibroblasts,” Journal of Tissue Science & Engineering, vol. S11, article 008, 2013. View at Publisher · View at Google Scholar
  34. K. Warstat, D. Meckbach, M. Weis-Klemm et al., “TGF-β enhances the integrin α2β1-mediated attachment of mesenchymal stem cells to type I collagen,” Stem Cells and Development, vol. 19, no. 5, pp. 645–656, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. T. Lanzicher, V. Martinelli, C. S. Long et al., “AFM single-cell force spectroscopy links altered nuclear and cytoskeletal mechanics to defective cell adhesion in cardiac myocytes with a nuclear lamin mutation,” Nucleus, vol. 6, no. 5, pp. 394–407, 2015. View at Publisher · View at Google Scholar
  36. N. Rodríguez-Fuentes, O. Reynoso-Ducoing, A. Rodríguez-Hernández et al., “Isolation of human mesenchymal stem cells and their cultivation on the porous bone matrix,” Journal of Visualized Experiments, vol. 96, pp. 1–7, 2015. View at Publisher · View at Google Scholar
  37. D. Salzig, A. Schmiermund, P. P. Grace, C. Elseberg, C. Weber, and P. Czermak, “Enzymatic detachment of therapeutic mesenchymal stromal cells grown on glass carriers in a bioreactor,” Open Biomedical Engineering Journal, vol. 7, no. 1, pp. 147–158, 2013. View at Publisher · View at Google Scholar · View at Scopus
  38. C. Weber, S. Pohl, R. Pörtner et al., “Expansion and harvesting of hMSC-TERT,” The Open Biomedical Engineering Journal, vol. 1, no. 1, pp. 38–46, 2007. View at Publisher · View at Google Scholar
  39. R. K. Das and O. F. Zouani, “A review of the effects of the cell environment physicochemical nanoarchitecture on stem cell commitment,” Biomaterials, vol. 35, no. 20, pp. 5278–5293, 2014. View at Publisher · View at Google Scholar · View at Scopus
  40. Y.-K. Wang and C. S. Chen, “Cell adhesion and mechanical stimulation in the regulation of mesenchymal stem cell differentiation,” Journal of Cellular and Molecular Medicine, vol. 17, no. 7, pp. 823–832, 2013. View at Publisher · View at Google Scholar · View at Scopus
  41. S. Gottipamula, M. S. Muttigi, U. Kolkundkar, and R. N. Seetharam, “Serum-free media for the production of human mesenchymal stromal cells: a review,” Cell Proliferation, vol. 46, no. 6, pp. 608–627, 2013. View at Publisher · View at Google Scholar · View at Scopus