Research Article
Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium
Table 1
Properties of the enzymes used for cell detachment.
| Detachment enzyme | Manufacturer | Incubation time (min) |
| Accutase | Sigma-Aldrich | 10 | Collagenase | PAA Laboratories GmbH | 60 | Prolyl-specific peptidase (PsP) | [23] | 40 | Trypsin | PAA | 10 |
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