Review Article
Biological Effects of Culture Substrates on Human Pluripotent Stem Cells
Table 1
Summary of culture substrates used for culturing undifferentiated hPSCs.
| Name | Commercial name (if any, only major) | Material type | References |
| Gelatinous protein mixture | Matrigel (Corning) | Crude extract secreted by EHS mouse sarcoma cells | [9, 28] | Geltrex (Thermo Fisher Scientific) | Cultrex BME (Trevigen) |
| Laminin | | Extracted protein | [9] |
| Laminin-511 | LN511 (Biolamina) | Recombinant protein | [29, 30] |
| Laminin-521 | LN521 (Biolamina) | Recombinant protein | [31, 32] | rhLaminin-521 (Thermo Fisher Scientific) |
| Laminin-E8 fragment | iMatrix-511 (Nippi) | Recombinant protein | [33, 34] |
| Vitronectin | Vitronectin XF (Stem Cell Technologies) | Extracted or recombinant protein | [35–38] |
| Truncated vitronectin | VTN-N (Thermo Fisher Scientific) | Recombinant protein | [39] |
| Fibronectin | | Extracted protein | [40–42] |
| Collagen type I | | Extracted protein | [20, 43] |
| Nanofibrous gelatin | | Processed gelatin | [44] |
| Customized spider silk protein | | Recombinant protein, containing vitronectin motif | [45] |
| Peptide-acrylate surfaces (PAS) | Synthemax (Corning) | Synthetic polymers with peptides | [46] |
| PMEDSAH | | Synthetic polymer | [47] |
| Synthetic substrates displaying heparin-binding peptides | | Synthetic polymers with peptides | [48] |
| Polyvinylalcohol-co-itaconic acid hydrogels grafted with aoligopeptide derived from vitronectin (KGGPQVTRGDVFTMP) | | Synthetic polymers with peptides | [49] |
| UV/ozone radiation | | Modification of typical cell culture plastics | [50] |
| Hydrogel interfaces of aminopropylmethacrylamide (APMAAm) | | Synthetic polymers | [51] |
| Human E-cadherin-Fc chimeric protein | | Recombinant protein | [31, 52] |
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