tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis

Figure 13

Immunofluorescent (IF) microscopic findings (400x) of neutrophils (CD11+ cells) in ischemic quadriceps at day 14 after critical limb ischemia (CLI) induction. (a–p) IF microscopic findings of neutrophils in ischemic area in four treatment conditions of four groups. (1) Statistical analysis for subgroups of each group. (1) In B B group, subgroups with different black symbols (, ), . (2) In M B group, subgroups with different black symbols (, ), . (3) In M M group, subgroups with different black symbols (, ), . (4) In B M group, subgroups with different black symbols (, ), . (2) Statistical analysis for group comparison with respective treatment condition. (1) In SC subgroup (red bar chart), . (2) In CLI subgroup (blue bar chart), groups with different blue symbols (, , and ), . (3) In SC + tPA subgroup (green bar chart), . (4) In CLI + tPA subgroup (orange bar chart), groups with different orange symbols (, , and ), . All statistical analyses were performed by one-way ANOVA followed by Bonferroni multiple comparison post hoc test. SC = sham control; tPA = tissue plasminogen activator. for each group.