Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells

Figure 1

Label-free imaging of umbilical cord tissue compartments. (a) Image composition derived from multiple fields of view for AF (red) and SHG (green) in unstained cord tissue, scale bar = 1 mm. (B–E) Detailed image sections of (a), scale bars = 20 μm. (b) Lamina intima and media of the umbilical vein. (c) Wharton’s jelly and perivascular zone of an umbilical artery. (d) Umbilical artery media and adventitia showing intensely fluorescent cells (arrows) and less bright smooth muscle cell bodies (arrowheads). (e) Subamnion and amniotic epithelial layer. (F) Detailed image of (e) displaying tripolar cells within the subamniotic zone, scale bar = 2 μm. Representative images from 3 independent donors are shown.

(a)

(b)

(c)

(d)

(e)

(f)