Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells

Figure 4

Marker-free visualization of Wharton’s jelly explant tissue and cell outgrowth. (a) Phase contrast image of an attached explant with outgrowing WJ-MSCs, scale bar = 200 μm. (b) AF (red) and SHG (green) of an attached explant (attachment site, bottom view), scale bar = 100 μm. (c) 2D image of cellular outgrowth from explant tissue at the attachment site (side view), scale bar = 50 μm. The images were derived from a Z-stack composition of the SHG and AF signals of (b). (d) Schematic view of the visualization plane of (c). Representative images for 3 independent experiments are shown.

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