Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells

Figure 5

TPM and SHG imaging of WJ-MSCs chondrogenic differentiation. (a) Alcian Blue staining of a chondrogenic pellet section, visualizing the nuclei (red) and chondrogenic matrix (blue), scale bar = 200 μm. (b–d) AF (red) and SHG (green) imaging of an intact chondrogenic pellet of ±1 mm diameter. (b) Chondrogenic pellet center, scale bar = 100 μm. (c) Cell and matrix nodule at the pellet border, scale bar = 20 μm. (d) Differentiated cells within their collagen rich matrix in the center of the pellet, scale bar = 20 μm. Representative images for 2 independent experiments are shown.

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