Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells

Figure 6

Autofluorescence originates from the perinuclear organelles in WJ-MSCs. (a) Bright field image of explant-derived WJ-MSCs. For reference, the cell boundary is delineated by a dotted white line and the nucleus is outlined by a full white line. (b) AF signal (red) and (c) SHG after two-photon excitation of the same cells. (d) Merged image. A perinuclear area of organelles is visible (view also the electronic Supplementary Material, Figure  S2). Scale bars = 20 μm.

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