Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes

<div>Differentiation of RLSCs towards hepatocytes correlates with early chromatin modifications on HNF4<i>α</i> promoter. qPCR analysis of ChIP assay performed to quantify H3K9Ac, H3K4me3, and H3K27me3, on the HNF1/HNF6 binding site of HNF4<i>α</i> promoter in RLSCs grown on plastic (CTRL) and on 0.4 kPa hydrogel for 24 and 48 hours. Amplification signals of specific immunoprecipitated samples (IP) and IgG are normalized versus total chromatin (Input) and expressed as % of Input. The mean ± SD of two independent experiments is shown. The upper part of the figure shows a schematic representation of murine HNF4<i>α</i> promoter indicating the binding site for HNF1/HNF6 and the relative positions of the qPCR primers.</div>

Stem Cells International

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Figure 4: Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes 

Figure 4 | Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes 