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Stem Cells International
Volume 2016 (2016), Article ID 5623235, 10 pages
Research Article

A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells

1Department of Neurosurgery, Brain Tumor Center, Erasmus MC, 3015 CE Rotterdam, Netherlands
2Department of Neurosurgery, St. Elisabeth Hospital, 5022 GC Tilburg, Netherlands

Received 8 October 2015; Revised 15 February 2016; Accepted 17 February 2016

Academic Editor: Heinrich Sauer

Copyright © 2016 A. Kleijn et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Table 1. Overview assays.

Supplemental Figure 1. The caspase 3/7 assay does not detect treatment effect in U373 and T98.

Supplemental Figure 2. Comparison of two cytotoxicity assays.

Supplemental Table 2. Characteristics of the used primary glioma stemElike cell cultures.

  1. Supplementary Material