Human Mesenchymal Stromal Cells from Different Sources Diverge in Their Expression of Cell Surface Proteins and Display Distinct Differentiation Patterns

Figure 4

Schematic draft of the experimental strategy to define and functionally characterize subsets of human bmMSCs. MSCs were sorted by fluorescence-activated cell sorting (FACS) (left panel). From the mononuclear cells the CD271+ subset, which is gated in R1, was further subdivided by staining with two additional antibodies to TNAP and CD56 positive cells (gates R2 and R3, middle panel). The populations defined by R2 or R3 were expanded in separate cultures and their proliferation and differentiation were compared (right panel) (for further details see [30]).