The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles

Figure 2

X chromosome CGIs profiles (promoter versus inside). (a) CGIs associated with gene inside; (b) CGIs associated with gene promoter. Each dot corresponds to a CGI in the X chromosome map; blue dots: CGIs represented in the array; red dots: CGI status after in vitro culture. axis: dots located at 0 correspond to islands that did not change the methylation status after culture; dots at 1: CGIs that reversed the methylation status from unmethylated to methylated after culture; dots at −1: CGIs that reversed the methylation status from methylated to unmethylated after culture. Light blue area defines a region of 18 Mb at Xp22.33-22.13 characterized by absence of de novo methylation in promoter CGIs.

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