The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles
Table 4
DNA methylation status of stemness-related genes in hBM-MSCs: pool of early passages versus pool of late passages.
Category
Genes
Cytoband
Gene region
Methylation status
Early
Late
Stemness Markers
FGF2 (bFGF)
4q26
Inside
LIF
22q12.2
Inside
SOX2
3q26.3
Inside
Downstream
TERT
5p15.33
Promoter
Inside
WNT3A
1q42
Inside
ZFP42
4q35.2
Promoter
MSC-Specific Markers
ALCAM
3q13.1
Inside
ANPEP
15q25
Promoter
BMP2
20p12
Inside
CASP3
4q34
Inside
CD44
11p13
Inside
ENG
9q34.11
Inside
ERBB2 (HER2)
17q12
Inside
FUT4
11q21
Inside
FZD9
7q11.23
Promoter
Inside
ITGA6
2q31.1
Inside
ITGAV
2q31-q32
Inside
KDR
4q11-q12
Promoter
MCAM
11q23.3
Promoter
Inside
NGFR
17q21-q22
Inside
NT5E
6q14-q21
Inside
PDGFRB
5q33.1
Inside
PROM1
4p15.32
Promoter
THY1
11q23.3
Promoter
Inside
Other Genes Associated with MSCs
ANXA5
4q27
Promoter
BDNF
11p13
Promoter
Inside
BMP7
20q13
Promoter
Inside
COL1A1
17q21.33
Promoter
CTNNB1
3p21
Inside
FUT1
19q13.3
Inside
GTF3A
13q12.3-q13.1
Inside
ICAM1
19p13.3-p13.2
Inside
ITGB1
10p11.2
Promoter
KITLG
12q22
Promoter
MMP2
16q13-q21
Inside
NES
1q23.1
Promoter
NUDT6
4q26
Inside
PIGS
17p13.2
Promoter
SLC17A5
6q13
Promoter
VEGFA
6p12
Inside
VIM
10p13
Inside
VWF
12p13.3
Inside
Red: the DNA methylated status prevails over the unmethylated one; green: the DNA unmethylated status prevails over the methylated one; red/green: balance between the two states.
