Stem Cells International

Research Article

The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles

Table 5

DNA methylation of differentiation-related genes in hBM-MSCs: pool of early passages versus pool of late passages.


Category	Genes	Cytoband	Gene region	Methylation status
Category	Genes	Cytoband	Gene region	Early	Late

MSC Differentiation Markers, osteogenesis	BMP2	20p12	Inside
	BMP6	6p24-p23	Inside
	HDAC1	1p34	Inside
	HNF1A	12q24.2	Inside
	KDR	4q11-q12	Promoter
	PTK2	8q24.3	Promoter
	RUNX2	6p21	Promoter
	RUNX2	6p21	Inside
	SMURF1	7q22.1	Promoter
	SMURF1	7q22.1	Inside
	SMURF2	17q22-q23	Promoter
	TBX5	12q24.1	Promoter
	TBX5	12q24.1	Inside

Adipogenesis	PPARG	3p25	Promoter
	RUNX2	6p21	Promoter
	RUNX2	6p21	Inside

Chondrogenesis	ABCB1 (MDR1)	7q21.12	Inside
	BMP2	20p12	Inside
	BMP4	14q22-q23	Promoter
			Inside
			Downstream
	BMP6	6p24-p23	Inside
	GDF5 (CDMP-1)	20q11.2	Inside
	GDF6	8q22.1	Promoter
	GDF6	8q22.1	Inside
	GDF7	2p24.1	Inside
	GDF7	2p24.1	Downstream
	HAT1	2q31.2-q33.1	Inside
	ITGAX	16p11.2	Inside
	KAT2B (PCAF)	3p24	Inside
	SOX9	17q23	Promoter
	SOX9	17q23	Inside
	TGFB1	19q13.1	Promoter
	TGFB1	19q13.1	Inside

Myogenesis	JAG1	20p12.1-p11.23	Promoter
	NOTCH1	9q34.3	Promoter
	NOTCH1	9q34.3	Inside

Tenogenesis	BMP2	20p12	Inside
	GDF15 (PLAB)	19p13.11	Inside
	SMAD4	18q21.1	Inside
	TGFB1	19q13.1	Promoter
	TGFB1	19q13.1	Inside

Red: the DNA methylated status prevails over the unmethylated one; green: the DNA unmethylated status prevails over the methylated one; red/green: balance between the two states.