Cellular Origins of Regenerating Nodules and  Malignancy in the FAH Model of Liver Injury after  Bone Marrow Cell Transplantation

<div>Reconstruction of liver functional units. Strong expression of <i>β</i>-Gal was detected in regenerating nodules by whole-mount X-Gal staining of FAH mutant livers (a) and expression of Fah in liver sections by Fah staining (b). Formation of remodeling liver functional units was observed in the regenerating nodules of Group 1 after BMT (c). (d) Higher power view of the indicated region in (d). (e) Intrahepatic biliary architecture in a liver section from a ROSAnZ control mouse. (f) Ductular structures in the regenerating nodules of a <svg height="12.5951pt" id="M29" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -12.3887 36.817 12.5951" width="36.817pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M606 650H137L129 618C217 609 221 609 203 518L129 129C115 46 106 40 26 32L18 0H300L307 32C210 40 203 45 218 129L249 305H358C454 305 455 296 457 219H491L531 425H497C465 353 463 350 366 350H259L300 569C306 605 310 611 346 611H444C522 611 535 598 541 578C550 557 553 519 552 485L588 489L606 650Z" id="g50-71"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="630" vert-adv-y="630"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,7.529,0)"><path d="M490 97L476 124C442 96 405 70 398 70C392 70 390 78 396 114C419 243 448 379 463 432L457 436C446 436 431 439 418 442C393 447 368 451 343 451C281 451 204 418 155 381C74 320 24 206 24 107C24 23 59 -12 88 -12C118 -12 155 5 191 34C236 70 290 122 328 177H330L312 84C296 0 311 -12 331 -12C355 -12 425 24 490 97ZM374 387C371 367 360 299 347 264C323 202 187 53 142 53C128 53 113 73 113 120C113 224 157 332 221 380C241 395 274 403 303 403C330 403 360 395 374 387Z" id="g50-98"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="514" vert-adv-y="514"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,14.214,0)"><path d="M509 90L493 118C465 91 427 70 418 70S409 77 415 103L469 327C494 429 466 451 436 451C412 451 387 444 356 427C307 400 222 342 160 264H158L246 667C253 701 252 710 243 710C228 710 169 680 87 673L83 644H117C155 644 160 640 149 588L24 -4L32 -12C57 -4 86 4 108 9L143 187C198 275 324 386 375 386C394 386 399 367 385 311L331 87C315 22 322 -12 351 -12C387 -12 452 24 509 90Z" id="g50-105"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="527" vert-adv-y="527"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,21.065,-5.872)"><path d="M556 236V289H56V236H556Z" id="g54-33"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="611" vert-adv-y="611"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,26.625,-5.872)"><path d="M381 704H317L48 -163H112L381 704Z" id="g50-48"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="429" vert-adv-y="429"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,30.532,-5.872)"><path d="M556 236V289H56V236H556Z" id="g54-33"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="611" vert-adv-y="611"></glyph.data></g></svg>-ROSAnZ mouse that received C57Bl/6J BMCs (Group 2). Scale bars are 60 <i>μ</i>m except for (a) (4 mm).</div>

Stem Cells International

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Figure 3

Figure 3: Cellular Origins of Regenerating Nodules and  Malignancy in the FAH Model of Liver Injury after  Bone Marrow Cell Transplantation 