Research Article

Cellular Origins of Regenerating Nodules and Malignancy in the FAH Model of Liver Injury after Bone Marrow Cell Transplantation

Figure 4

Identification of the cellular origins in liver tumors. Images of gross liver specimens with a tumor (a) and immunofluorescence detection of donor BMC-derived Gfp+ nodules (b) in 8-month-old mice of Group 3 as shown in (a) and (b). Expression of Gfp (green) (c) and β-Gal (red) (d) was observed in tumor sections. No coexpression of Gfp and anti-β-Gal was found in the tumor (e). Serial liver sections were stained with antibodies against Afp (tumor marker; red) (f) and the host marker (β-Gal; red). Afp-positive cells confirmed hepatic carcinoma. A higher magnification of the tumor cells stained for Afp revealed that the donor-derived hepatocytes expressing Gfp (green) were negative for Afp (red) (g). Scale bars are 100 μm except for (a) and (b) (4 mm).
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