Research Article

Cellular Origins of Regenerating Nodules and Malignancy in the FAH Model of Liver Injury after Bone Marrow Cell Transplantation

Figure 5

Histochemical characteristic of liver tumors. Liver sections from a 32-week mouse after BMT were stained for Fah (brown) and BrdU (dark nuclei) by immunohistochemistry to indicate the region of Fah+ nodules with BrdU uptake (a). The higher power view of the indicated region shows a Fah tumor with significant BrdU uptake in (b). Sections were also stained with hematoxylin and eosin to identify malignant cells (c and d) and Sirius Red to detect collagen (e). Sections (b) and (c) were serial sections and vascular invasion was also observed in (e). Scale bars are 100 μm.