Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Figure 4

Effects of CIP on Wnt/β-catenin signaling, EMT, and self-renewal transcription factors in DPCs. (a) Cells were treated with CIP (1–10 μg/mL) for 72 h. After indicated treatment, the levels of Wnt/β-catenin signaling (Akt, p-Akt (Ser 473), GSK3β, p-GSK3β (Ser 9), and β-catenin) were analyzed by western blot analysis. The immunoblot signals were quantified by densitometry and mean data from independent experiments were normalized to the results. (b) EMT and self-renewal transcription factors (ZEB1, Oct-4, Nanog, Slug, and Snail) were determined by western blot analysis. (c) Downstream targets of Snail including N-cadherin, vimentin, total Erk, and p-Erk (Thr 202/Tyr 204) were analyzed by western blot analysis. β-actin was used as the loading control. The western blot signals were quantified by densitometry and mean data from independent experiments were normalized to the results. The data represent the means of four independent samples ± SD. versus untreated control.

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