Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes

Figure 4

Characterization of germ cell-derived colonies in subculture. The GDC cells in passages 2 and 11 were stained with antibodies against PGP 9.5 and GFRα-1 ((a) and (b)), while the nuclei were stained with DAPI. Expression of germ and stem cell markers is shown in panel (c). The proportion of GFRα-1-positive cells, analyzed in passages 0 and 8, is shown in panel (d). Scale bars indicate 10 μm in panels of (a) and (b).

(a)

(b)

(c)

(d)