Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes

Figure 5

Subculture of frozen germ cell-derived colonies. The cells from frozen colonies, which were labeled with red trackers in passage 13, were maintained in the colonies of passage 14 ((A), (B), and (C)). Panels (D), (E), and (F) show the bright-field images of panels (A), (B), and (C), respectively. Scale bars indicate 100 μm in all the panels. GDC: germ cell-derived colony.